Stem Cell and Tissue Engineering (Master) (with Thesis) | |||||
Master | TR-NQF-HE: Level 7 | QF-EHEA: Second Cycle | EQF-LLL: Level 7 |
Course Code: | KHY5012 | ||||
Course Name: | Molecular Biological Techniques 2 | ||||
Semester: |
Fall Spring |
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Course Credits: |
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Language of instruction: | Turkish | ||||
Course Condition: | |||||
Does the Course Require Work Experience?: | No | ||||
Type of course: | Departmental Elective | ||||
Course Level: |
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Mode of Delivery: | Face to face | ||||
Course Coordinator: | Dr. Öğr. Üy. AYŞE KÖYLÜ | ||||
Course Lecturer(s): | Özgür Tataroğlu | ||||
Course Assistants: |
Course Objectives: | This course covers the theory and practice of molecular biology techniques used in stem cell and tissue engineering. The aim of the course is to give students the necessary background to use these techniques in their thesis. |
Course Content: | The methods used in molecular biological analyzes will be emphasized. The course covers both theoretical and practical stages. |
The students who have succeeded in this course;
1) Can analyze DNA, RNA and protein 2) Understands the molecular biological mechanisms inside the cell 3) Comprehends the methods used in synthetic biology 4) Gains practice for laboratory studies |
Week | Subject | Related Preparation |
1) | Introduction: Structure, regulation and expression of DNA, RNA and proteins | |
2) | Gene design and plasmid vectors | |
3) | Molecular cloning 1 (PCR gene amplification) | |
4) | Molecular cloning 2 (Fragment analysis and isolation from agarose gel) | |
5) | Molecular cloning 3 (DNA sequencing and cloning validation) | |
6) | Bacterial culture 1 (clone selection and selection) | |
7) | Bacterial culture 2 (production and isolation of DNA with purity and amount suitable for transfection) | |
8) | Methods of gene transfer into cells | |
9) | Transfection efficiency measurement 1 (western blot, ELISA, enzymatic reactions) | |
10) | Transfection efficiency measurement 2 (immune staining and fluorescence microscopy) | |
11) | Live cell imaging | |
12) | Protein discovery 1 (isoelectric point and 2 dimensional gel electrophoresis) | |
13) | Protein discovery 2 (Mass spectrometry and high pressure liquid chromatography) | |
14) | Protein discovery 3 (biological screens) |
Course Notes / Textbooks: | |
References: |
Course Learning Outcomes | 1 |
2 |
3 |
4 |
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Program Outcomes | ||||||||||||||||
1) Being able to develop and deepen their knowledge in the same or a different field at the level of expertise, based on their undergraduate level qualifications. | ||||||||||||||||
2) To be able to comprehend the interdisciplinary interaction with which the field is related. | ||||||||||||||||
3) To be able to use the theoretical and applied knowledge at the level of expertise acquired in the field. | ||||||||||||||||
4) To be able to interpret and create new knowledge by integrating the knowledge gained in the field with the knowledge from different disciplines, | ||||||||||||||||
5) To be able to solve the problems encountered in the field by using research methods. | ||||||||||||||||
6) Being able to independently carry out a work that requires expertise in the field. | ||||||||||||||||
7) To be able to develop new strategic approaches for the solution of complex and unpredictable problems encountered in applications related to the field and to produce solutions by taking responsibility. | ||||||||||||||||
8) To be able to lead in environments that require the resolution of problems related to the field. | ||||||||||||||||
9) To be able to critically evaluate the knowledge and skills acquired in the field of expertise and to direct their learning. | ||||||||||||||||
10) To be able to systematically transfer the current developments in the field and their own studies to groups in and outside the field, in written, verbal and visual forms, by supporting them with quantitative and qualitative data. | ||||||||||||||||
11) To be able to critically examine and develop social relations and the norms that guide these relations, and to take action to change them when necessary. | ||||||||||||||||
12) To be able to communicate orally and in writing using a foreign language at least at the B2 General Level of the European Language Portfolio. | ||||||||||||||||
13) To be able to use information and communication technologies at an advanced level, together with computer software at the level required by the field. | ||||||||||||||||
14) To be able to supervise and teach these values by observing social, scientific, cultural and ethical values in the stages of collecting, interpreting, applying and announcing the data related to the field. | ||||||||||||||||
15) To be able to develop strategy, policy and implementation plans on issues related to his field and to evaluate the results obtained within the framework of quality processes. | ||||||||||||||||
16) To be able to use the knowledge, problem solving and/or application skills they have internalized in the field, in interdisciplinary studies. |
No Effect | 1 Lowest | 2 Average | 3 Highest |
Program Outcomes | Level of Contribution | |
1) | Being able to develop and deepen their knowledge in the same or a different field at the level of expertise, based on their undergraduate level qualifications. | 3 |
2) | To be able to comprehend the interdisciplinary interaction with which the field is related. | 3 |
3) | To be able to use the theoretical and applied knowledge at the level of expertise acquired in the field. | 3 |
4) | To be able to interpret and create new knowledge by integrating the knowledge gained in the field with the knowledge from different disciplines, | 3 |
5) | To be able to solve the problems encountered in the field by using research methods. | 3 |
6) | Being able to independently carry out a work that requires expertise in the field. | 3 |
7) | To be able to develop new strategic approaches for the solution of complex and unpredictable problems encountered in applications related to the field and to produce solutions by taking responsibility. | 3 |
8) | To be able to lead in environments that require the resolution of problems related to the field. | 3 |
9) | To be able to critically evaluate the knowledge and skills acquired in the field of expertise and to direct their learning. | 3 |
10) | To be able to systematically transfer the current developments in the field and their own studies to groups in and outside the field, in written, verbal and visual forms, by supporting them with quantitative and qualitative data. | 3 |
11) | To be able to critically examine and develop social relations and the norms that guide these relations, and to take action to change them when necessary. | 3 |
12) | To be able to communicate orally and in writing using a foreign language at least at the B2 General Level of the European Language Portfolio. | 3 |
13) | To be able to use information and communication technologies at an advanced level, together with computer software at the level required by the field. | 3 |
14) | To be able to supervise and teach these values by observing social, scientific, cultural and ethical values in the stages of collecting, interpreting, applying and announcing the data related to the field. | 3 |
15) | To be able to develop strategy, policy and implementation plans on issues related to his field and to evaluate the results obtained within the framework of quality processes. | 3 |
16) | To be able to use the knowledge, problem solving and/or application skills they have internalized in the field, in interdisciplinary studies. | 3 |
Semester Requirements | Number of Activities | Level of Contribution |
Homework Assignments | 1 | % 100 |
total | % 100 | |
PERCENTAGE OF SEMESTER WORK | % 100 | |
PERCENTAGE OF FINAL WORK | % | |
total | % 100 |
Activities | Number of Activities | Preparation for the Activity | Spent for the Activity Itself | Completing the Activity Requirements | Workload | ||
Application | 1 | 0 | 55 | 55 | |||
Final | 1 | 0 | 60 | 60 | |||
Total Workload | 115 |