Stem Cell and Tissue Engineering (Master) (with Thesis) Preview
Master	TR-NQF-HE: Level 7	QF-EHEA: Second Cycle	EQF-LLL: Level 7

Course Introduction and Application Information

Course Code:

KHY5002

Course Name:

Molecular Biological Techniques 1

Semester:

Fall
Spring

Course Credits:

ECTS

Language of instruction:

Turkish

Course Condition:

Does the Course Require Work Experience?:

Type of course:

Departmental Elective

Course Level:

Master

TR-NQF-HE:7. Master`s Degree

QF-EHEA:Second Cycle

EQF-LLL:7. Master`s Degree

Mode of Delivery:

Face to face

Course Coordinator:

Dr. Öğr. Üy. AYŞE KÖYLÜ

Course Lecturer(s):

Özgür Tataroğlu

Course Assistants:

Course Objective and Content

Course Objectives:	This course covers the theory and practice of molecular biology techniques used in stem cell and tissue engineering. The aim of the course is to give students the necessary background to use these techniques in their thesis.
Course Content:	Among the methods used in molecular biological analysis, protein/antigen based analysis methods such as immunohistochemistry, immunofluorescent staining, ELISA and western blot will be emphasized. The course covers both theoretical and practical stages.

Learning Outcomes

The students who have succeeded in this course;
1) Can analyze DNA, RNA and protein
2) Understands the molecular biological mechanisms inside the cell
3) Comprehends the methods used in synthetic biology
4) Gains practice for laboratory studies

Course Flow Plan

Week	Subject	Related Preparation
1)	Introduction to Molecular Biology
2)	DNA structure
3)	DNA regulation
4)	Structure and regulation of RNA
5)	RNA expression
6)	Protein synthesis, structure and regulation
7)	Synthetic DNA cloning
8)	DNA isolation and quantification techniques
9)	Agarose gel electrophoresis
10)	Polymerase chain reaction and primer design
11)	Quantitative Polymerase chain reaction and RNA reverse transcription
12)	Protein isolation methods
13)	Protein analysis methods
14)	Western blot, ELISA, antibody-antigen tests

Sources

Course Notes / Textbooks:
References:

Course - Program Learning Outcome Relationship

Course Learning Outcomes	1	2	3	4
Program Outcomes
1) Being able to develop and deepen their knowledge in the same or a different field at the level of expertise, based on their undergraduate level qualifications.
2) To be able to comprehend the interdisciplinary interaction with which the field is related.
3) To be able to use the theoretical and applied knowledge at the level of expertise acquired in the field.
4) To be able to interpret and create new knowledge by integrating the knowledge gained in the field with the knowledge from different disciplines,
5) To be able to solve the problems encountered in the field by using research methods.
6) Being able to independently carry out a work that requires expertise in the field.
7) To be able to develop new strategic approaches for the solution of complex and unpredictable problems encountered in applications related to the field and to produce solutions by taking responsibility.
8) To be able to lead in environments that require the resolution of problems related to the field.
9) To be able to critically evaluate the knowledge and skills acquired in the field of expertise and to direct their learning.
10) To be able to systematically transfer the current developments in the field and their own studies to groups in and outside the field, in written, verbal and visual forms, by supporting them with quantitative and qualitative data.
11) To be able to critically examine and develop social relations and the norms that guide these relations, and to take action to change them when necessary.
12) To be able to communicate orally and in writing using a foreign language at least at the B2 General Level of the European Language Portfolio.
13) To be able to use information and communication technologies at an advanced level, together with computer software at the level required by the field.
14) To be able to supervise and teach these values by observing social, scientific, cultural and ethical values in the stages of collecting, interpreting, applying and announcing the data related to the field.
15) To be able to develop strategy, policy and implementation plans on issues related to his field and to evaluate the results obtained within the framework of quality processes.
16) To be able to use the knowledge, problem solving and/or application skills they have internalized in the field, in interdisciplinary studies.

Course - Learning Outcome Relationship

No Effect	1 Lowest	2 Average	3 Highest

	Program Outcomes	Level of Contribution
1)	Being able to develop and deepen their knowledge in the same or a different field at the level of expertise, based on their undergraduate level qualifications.	3
2)	To be able to comprehend the interdisciplinary interaction with which the field is related.	3
3)	To be able to use the theoretical and applied knowledge at the level of expertise acquired in the field.	3
4)	To be able to interpret and create new knowledge by integrating the knowledge gained in the field with the knowledge from different disciplines,	3
5)	To be able to solve the problems encountered in the field by using research methods.	3
6)	Being able to independently carry out a work that requires expertise in the field.	3
7)	To be able to develop new strategic approaches for the solution of complex and unpredictable problems encountered in applications related to the field and to produce solutions by taking responsibility.	3
8)	To be able to lead in environments that require the resolution of problems related to the field.	3
9)	To be able to critically evaluate the knowledge and skills acquired in the field of expertise and to direct their learning.	3
10)	To be able to systematically transfer the current developments in the field and their own studies to groups in and outside the field, in written, verbal and visual forms, by supporting them with quantitative and qualitative data.	3
11)	To be able to critically examine and develop social relations and the norms that guide these relations, and to take action to change them when necessary.	3
12)	To be able to communicate orally and in writing using a foreign language at least at the B2 General Level of the European Language Portfolio.	3
13)	To be able to use information and communication technologies at an advanced level, together with computer software at the level required by the field.	3
14)	To be able to supervise and teach these values by observing social, scientific, cultural and ethical values in the stages of collecting, interpreting, applying and announcing the data related to the field.	3
15)	To be able to develop strategy, policy and implementation plans on issues related to his field and to evaluate the results obtained within the framework of quality processes.	3
16)	To be able to use the knowledge, problem solving and/or application skills they have internalized in the field, in interdisciplinary studies.	3

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Homework Assignments	1	% 100
total		% 100
PERCENTAGE OF SEMESTER WORK		% 100
PERCENTAGE OF FINAL WORK		%
total		% 100

Workload and ECTS Credit Calculation

Activities	Number of Activities	Preparation for the Activity	Spent for the Activity Itself	Completing the Activity Requirements	Workload
Application	1	55			55
Final	1	60			60
Total Workload					115