| Medical Biology and Genetics (DR) | |||||
| PhD | TR-NQF-HE: Level 8 | QF-EHEA: Third Cycle | EQF-LLL: Level 8 | ||
| Course Code: | TGY6014 | ||||
| Course Name: | Molecular Techniques and Application Areas | ||||
| Semester: | Fall | ||||
| Course Credits: |
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| Language of instruction: | Turkish | ||||
| Course Condition: | |||||
| Does the Course Require Work Experience?: | No | ||||
| Type of course: | Departmental Elective | ||||
| Course Level: |
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| Mode of Delivery: | Face to face | ||||
| Course Coordinator: | Dr. Öğr. Üy. MURADİYE ACAR | ||||
| Course Lecturer(s): | MURADİYE ACAR | ||||
| Course Assistants: |
| Course Objectives: | THE AIM OF THIS COURSE IS TO GAIN INFORMATION ABOUT THE MOLECULAR TECHNIQUES USED IN THE DIAGNOSIS OF GENETIC DISEASES, TO LEARN THE WORKING PRINCIPLES OF THE METHODS, TO KNOW THE EXPERIMENTAL PROCEDURE STAGES AND TO EVALUATE THE OBTAINED DATA. |
| Course Content: | USE OF PHENOL CHLOROFORM METHOD IN DNA ISOLATION, USE OF SPIN COLUMN METHOD IN DNA ISOLATION, USE OF MAGNETIC BEADS IN DNA ISOLATION, PCR AND MOST USED PCR TYPES, AGAROSE GEL ELECTROPHORESIS METHOD, SANGER SEQUENCE METHOD AND ITS AREAS OF USE, RFLP METHOD AND ITS USE AREAS, RNA TRACE PHENOL CHLOROFORM AND USE OF SPIN COLUMN METHOD, CDNA SYNTHESIS AND REAL TIME PCR TECHNIQUES AND AREAS OF USE, USE OF ACGH AND MLPA TECHNIQUES IN GENETIC DIAGNOSIS, NEW GENERATION SEQUENCING METHODS AND COMPARISON OF ALL SEQUENCING METHODS, METHODS USED IN MIRNA ISOLATION |
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The students who have succeeded in this course;
1) EXPLAIN THE SCIENTIFIC LOGIC AND STEPS OF DNA ISOLATION USING THE PHENOL CHLOROFORM METHOD. 2) EXPLAIN THE SCIENTIFIC LOGIC AND STAGES OF DNA ISOLATION USING THE SPIN COLUMN METHOD. 3) KNOW THE SCIENTIFIC LOGIC AND STEPS OF DNA ISOLATION WITH MAGNETIC BEADS. 4) COMPARE THE BASIC PRINCIPLE OF PCR AND CURRENTLY USED PCR TYPES. 5) EXPLAIN THE STEPS OF THE AGAROSE GEL ELECTROPHORESIS METHOD 6) KNOW THE SANGER SEQUENCE METHOD AND ITS AREAS OF USE 7) CAN EXPLAIN THE USED AREAS OF THE RFLP METHOD 8) EXPLAIN THE STEPS OF PHENOL CHLOROFORM AND SPIN COLUMN METHOD IN RNA ISOLATION 9) KNOW CDNA SYNTHESIS AND REAL TIME PCR TECHNIQUES AND AREAS OF USE 10) EXPLAIN THE USE OF ACGH AND MLPA TECHNIQUES IN GENETIC DIAGNOSIS 11) NEW GENERATION SEQUENCING METHODS AND COMPARE ALL SEQUENCING METHODS 12) IDENTIFY THE METHODS USED IN MIRNA ISOLATION |
| Week | Subject | Related Preparation |
| 1) | USING THE PHENOL CHLOROFORM METHOD IN DNA ISOLATION | |
| 2) | USING THE SPIN COLUMN METHOD IN DNA ISOLATION | |
| 3) | USE OF MAGNETIC BEADS IN DNA ISOLATION | |
| 4) | PCR AND MOST USED PCR TYPES | |
| 5) | AGAROSE GEL ELECTROPHORESIS METHOD | |
| 6) | SANGER SEQUENCE METHOD AND AREAS OF USE | |
| 7) | RFLP METHOD AND AREAS OF USE | |
| 8) | MID TERM | |
| 9) | USE OF PHENOL CHLOROFORM AND SPIN COLUMN METHOD IN RNA ISOLATION | |
| 10) | cDNA SYNTHESIS AND REAL TIME PCR TECHNIQUE AND USAGE AREAS | |
| 11) | USE OF ACGH AND MLPA TECHNIQUES IN GENETIC DIAGNOSIS | |
| 12) | NEW GENERATION SEQUENCING METHODS AND COMPARISON OF ALL SEQUENCING METHODS | |
| 13) | METHODS USED IN MIRNA ISOLATION | |
| 14) | FİNAL EXAM |
| Course Notes / Textbooks: | ÖĞRENCİNİN HAZIRLADIĞI SUNUMLAR |
| References: | ÖĞRENCİNİN HAZIRLADIĞI SUNUMLAR |
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| Program Outcomes | ||||||||||||
| 1) Follows the current literature in the field of Medical Biology and Genetics, collects information, analyzes and synthesizes it with appropriate evaluation methods and guides clinical practices. | ||||||||||||
| 2) Uses appropriate methods and technological structures for scientific research, establishes the appropriate hypothesis, and determines research methods. | ||||||||||||
| 3) Interprets the results obtained as a result of scientific research, transfers and develops the evidence-based knowledge obtained into applications, presents its results at national and international scientific meetings and publishes them in peer-reviewed journals. | ||||||||||||
| 4) Realizes the principles of lifelong learning by observing professional autonomy within the ethical, deontological and legal framework in Medical Biology and Genetics applications and scientific studies. | ||||||||||||
| 5) Provides interdisciplinary collaboration in academic and clinical environments and has knowledge and skills in problem solving and management. | ||||||||||||
| 6) Uses the information obtained from scientific research and literature for the benefit of society and ensures the shaping of health policies necessary to improve public health and takes part in these policies. | ||||||||||||
| 7) Trains biologists who combine clinical experience and academic knowledge, produce, plan and implement creative solutions in the field of medical biology and have qualified knowledge and skills. | ||||||||||||
| No Effect | 1 Lowest | 2 Average | 3 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | Follows the current literature in the field of Medical Biology and Genetics, collects information, analyzes and synthesizes it with appropriate evaluation methods and guides clinical practices. | 2 |
| 2) | Uses appropriate methods and technological structures for scientific research, establishes the appropriate hypothesis, and determines research methods. | |
| 3) | Interprets the results obtained as a result of scientific research, transfers and develops the evidence-based knowledge obtained into applications, presents its results at national and international scientific meetings and publishes them in peer-reviewed journals. | |
| 4) | Realizes the principles of lifelong learning by observing professional autonomy within the ethical, deontological and legal framework in Medical Biology and Genetics applications and scientific studies. | |
| 5) | Provides interdisciplinary collaboration in academic and clinical environments and has knowledge and skills in problem solving and management. | |
| 6) | Uses the information obtained from scientific research and literature for the benefit of society and ensures the shaping of health policies necessary to improve public health and takes part in these policies. | |
| 7) | Trains biologists who combine clinical experience and academic knowledge, produce, plan and implement creative solutions in the field of medical biology and have qualified knowledge and skills. | 1 |
| Semester Requirements | Number of Activities | Level of Contribution |
| Presentation | 1 | % 40 |
| Midterms | 1 | % 30 |
| Final | 1 | % 30 |
| total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 70 | |
| PERCENTAGE OF FINAL WORK | % 30 | |
| total | % 100 | |
| Activities | Number of Activities | Workload |
| Course Hours | 14 | 42 |
| Presentations / Seminar | 13 | 39 |
| Homework Assignments | 13 | 39 |
| Midterms | 1 | 3 |
| Final | 1 | 3 |
| Total Workload | 126 | |