ISTINYE UNIVERSITY BİLGİ PAKETİ / DERS KATALOĞU
| Histology and Embryology (DR) | |||||
| PhD | TR-NQF-HE: Level 8 | QF-EHEA: Third Cycle | EQF-LLL: Level 8 | ||
Course Introduction and Application Information
| Course Code: | HİS6107 | ||||
| Course Name: | Basic Histochemical Techniques | ||||
| Semester: | Fall | ||||
| Course Credits: |
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| Language of instruction: | Turkish | ||||
| Course Condition: | |||||
| Does the Course Require Work Experience?: | Yes | ||||
| Type of course: | Compulsory Courses | ||||
| Course Level: |
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| Mode of Delivery: | Face to face | ||||
| Course Coordinator: | Dr. Öğr. Üy. AYŞE KÖYLÜ | ||||
| Course Lecturer(s): | Dr. Ayşe Köylü | ||||
| Course Assistants: |
Course Objective and Content
| Course Objectives: | To teach the basic techniques used in histology laboratories and to gain skills by practicing these techniques at the end of the course. It is to enable the student to independently experience the process of preparing preparations from tissue. |
| Course Content: | (LESSON 1) Fixation types and methods 1 Purpose of fixation, changes in tissues as a result of fixation, types of fixation, working mechanism of fixation, factors affecting fixation (LESSON 2) Fixation types and methods 2 Formaldehyde, glutaraldehyde, osmium tetraoxide, methyl alcohol, acetone, Bouin's fixative, Mercury, Zenker types and their properties; fixation artifacts (LESSON 3) Tissue tracking steps Purpose of tissue tracking, e.g. size characteristics, dehydration, dehydration agents, clearing, clearing agents, impregnation, machine tracking, manual tracking procedure examples and points to consider (LESSON 4) Dyeing types and principles Dye types, theory of dyeing, factors affecting dyeing, metachromatic dyes, mordant, dyeing procedure examples and points to consider. (LESSON 5) Hematoxylin and eosin stain Hematoxylin types, hematoxylin and eosin staining procedure examples and points to consider. Interpretation of sample micrografts (LESSON 6) Carbohydrate, protein, lipid dyes 1 Staining of different carbohydrates, Periodic Acid-Schiff's (PAS) dye and its properties, Alcian blue dye and its properties. Interpretation of sample micrografts (LESSON 7) Carbohydrate, protein, lipid dyes 2 Different lipid dyes, Sudan black, Oil red O dye and its properties, different protein dyes. Interpretation of sample micrografts (LESSON 8) Nucleic acid and pigment dyes Nucleic acid and different pigment (hemosiderin, bile, melanin, lipofuscin, chromaffin) dyes, examples and properties. Interpretation of sample micrografts (LESSON 9) Connective tissue dyes 1 Masson Trichrome, Van Gieson, Verhoeff's paints and their properties. Interpretation of sample micrografts (LESSON 10) Connective tissue dyes 2 Orcein, Phosphotungstic Acid, Weigert dyes and their properties. Interpretation of sample micrografts (LESSON 11) Immunohistochemistry 1 Working principle and method of immunohistochemical dyes (tissue procurement, fixation, antigen retrieval, use of positive and negative controls, chromogen staining) (LESSON 12) Immunohistochemistry 2 Handling and managing “Troubleshooting”, quality controls, factors affecting staining quality, observing the preparations on the monitor and points to pay attention to (LESSON 13) Immunohistochemistry 3 Observation and interpretation of micrograft images of different immunohistochemical markers |
Learning Outcomes
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The students who have succeeded in this course;
1) • Classifies the dyes used in histology 2) • Gives examples of dyes used in histology 3) • Interprets a micrograft 4) • Applies immunohistochemical steps 5) • Applies histochemical steps 6) • Provides solutions to problems that may arise during applications |
Course Flow Plan
| Week | Subject | Related Preparation |
| 1) | Fiksasyon çeşitleri ve yöntemleri 1 | |
| 2) | Fiksasyon çeşitleri ve yöntemleri 2 | |
| 3) | Doku takip basamakları | |
| 4) | Boyama çeşitleri ve prensipleri | |
| 5) | Hematoksilen ve eozin boyası | |
| 6) | Karbonhidrat, protein, lipid boyaları 1 | |
| 7) | Karbonhidrat, protein, lipid boyaları 2 | |
| 8) | Nükleik asit ve pigment boyaları | |
| 9) | exam | |
| 10) | Bağ dokusu boyaları 1 | |
| 11) | Bağ dokusu boyaları 2 | |
| 12) | İmmünohistokimya 1 | |
| 13) | İmmünohistokimya 2 | |
| 14) | İmmünohistokimya 3 | |
| 15) | Final |
Sources
| Course Notes / Textbooks: | • S. Kim Suvarna, Christopher Layton, John D. Bancroft. Bancroft’s Theory and Practice of Histological Techniques. 8th ed. • Pranab Dey. Basic and Advanced Laboratory Techniques in Histopathology and Cytology. Springer Nature 2018. |
| References: | S. Kim Suvarna, Christopher Layton, John D. Bancroft. Bancroft’s Theory and Practice of Histological Techniques. 8th ed. • Pranab Dey. Basic and Advanced Laboratory Techniques in Histopathology and Cytology. Springer Nature 2018. |
Course - Program Learning Outcome Relationship
| Course Learning Outcomes | 1 |
2 |
3 |
4 |
5 |
6 |
|---|---|---|---|---|---|---|
| Program Outcomes | ||||||
| 1) Be able to comprehend the interdisciplinary interaction with which the field is related; Ability to reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | 2 | 2 | 3 | 3 | 2 | 2 |
| 2) Be able to evaluate and use new information in the field with a systematic approach. | 3 | 2 | 3 | 3 | 2 | 2 |
| 3) Be able to examine social relations and the norms that guide these relations from a critical perspective, develop them and manage actions to change them when necessary. | 2 | 3 | 2 | 2 | 3 | 3 |
| 4) Be able to develop new ideas and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | 2 | 2 | 3 | 2 | 2 | 3 |
| 5) Be able to contribute to the process of the society in which one lives in becoming and sustaining an information society by introducing scientific, technological, social or cultural advances in the field. | 2 | 2 | 2 | 2 | 3 | 2 |
| 6) Be able to contribute to progress in the field by independently carrying out an original study that brings innovation to the field, develops a new idea, method, design and/or application, or applies a known idea, method, design and/or application to a different field. | 2 | 2 | 2 | 3 | 2 | 2 |
Course - Learning Outcome Relationship
| No Effect | 1 Lowest | 2 Average | 3 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | Be able to comprehend the interdisciplinary interaction with which the field is related; Ability to reach original results by using knowledge that requires expertise in analyzing, synthesizing and evaluating new and complex ideas. | 3 |
| 2) | Be able to evaluate and use new information in the field with a systematic approach. | 3 |
| 3) | Be able to examine social relations and the norms that guide these relations from a critical perspective, develop them and manage actions to change them when necessary. | 3 |
| 4) | Be able to develop new ideas and methods related to the field by using high-level mental processes such as creative and critical thinking, problem solving and decision making. | 3 |
| 5) | Be able to contribute to the process of the society in which one lives in becoming and sustaining an information society by introducing scientific, technological, social or cultural advances in the field. | 3 |
| 6) | Be able to contribute to progress in the field by independently carrying out an original study that brings innovation to the field, develops a new idea, method, design and/or application, or applies a known idea, method, design and/or application to a different field. | 3 |
Assessment & Grading
| Semester Requirements | Number of Activities | Level of Contribution |
| Midterms | 1 | % 40 |
| Final | 1 | % 60 |
| total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 40 | |
| PERCENTAGE OF FINAL WORK | % 60 | |
| total | % 100 | |
Workload and ECTS Credit Calculation
| Activities | Number of Activities | Workload |
| Course Hours | 13 | 26 |
| Laboratory | 3 | 3 |
| Study Hours Out of Class | 13 | 103 |
| Midterms | 1 | 2 |
| Final | 1 | 3 |
| Total Workload | 137 | |