Medical Laboratory Techniques Preview
Associate	TR-NQF-HE: Level 5	QF-EHEA: Short Cycle	EQF-LLL: Level 5

Course Introduction and Application Information

Course Code:

TLT026

Course Name:

Molecular Biology Techniques

Semester:

Spring

Course Credits:

ECTS

Language of instruction:

Turkish

Course Condition:

Does the Course Require Work Experience?:

Type of course:

Departmental Elective

Course Level:

Associate

TR-NQF-HE:5. Master`s Degree

QF-EHEA:Short Cycle

EQF-LLL:5. Master`s Degree

Mode of Delivery:

E-Learning

Course Coordinator:

Dr. Öğr. Üy. FATMA KÜBRA TOMBULTÜRK

Course Lecturer(s):

Fatma Kübra Tombultürk

Course Assistants:

Course Objective and Content

Course Objectives:	This course teaches each student the structure, properties, isolation and purity and quantification methods of biomolecules; to gain the ability to apply electrophoresis, immunoassay, blotting and flow cytometry methods, which are other basic techniques applied in molecular biology laboratories; It aims to teach mutation analysis methods.
Course Content:	Teaching the tasks and functions of the molecular biology laboratory and the tools and equipment used in this laboratory, the rules to be followed, the structure and properties of biomolecules, the tests applied in molecular biology and mutation analysis techniques.

Learning Outcomes

The students who have succeeded in this course;
1) Recognition of molecular biology laboratory
2) Understanding the functioning of the molecular biology laboratory and the safety precautions to be followed
3) To have general knowledge about biomolecules
4) To have knowledge about isolation, purity and quantification of biomolecules.
5) Acquiring theoretical and practical information about PCR and Electrophoresis techniques
6) Learning the molecular biological techniques used in mutation analysis
7) Acquiring theoretical and practical knowledge about immunoassay, blotting and flow cytometry techniques

Course Flow Plan

Week	Subject	Related Preparation
1)	Introduction to the Course, Aim of the Course and Learning Objectives
2)	Introduction to Molecular Biology
3)	Functioning, Duties and Rules of the Molecular Biology Laboratory
4)	Structure and Properties of Biomolecules: DNA, RNA and Proteins
5)	Protein Isolation Techniques
6)	Nucleic Acid Isolation Techniques
7)	Purity and Quantity Determination of Biomolecules
8)	Midterm exam
9)	Polymerase Chain Reaction (PCR) - Electrophoresis Techniques
10)	Mutation Analysis Methods: RFLP and Sequence Analysis Techniques
11)	Mutation Analysis Methods: Real-Time PCR
12)	Immuno Assay Techniques
13)	Blotting Methods
14)	Flow Cytometer (Flowing Cell Meter)
15)	Final exam
16)	Final exam

Sources

Course Notes / Textbooks:	Öğretim üyesinin ders notları Laboratuvar Dünyası - Uzay GÖRMÜŞ
References:	Academic member's lecture notes

Course - Program Learning Outcome Relationship

Course Learning Outcomes	1	2	3	4	5	6	7
Program Outcomes
1) Medical laboratory techniques apply the basic knowledge and skills on the path to diagnosis from the patient samples, which are deemed necessary by the physicians, by gaining the competence towards the general and applied sciences of basic medicine fields such as microbiology, biochemistry, hematology and immunology for human health.
2) It takes part in the acceptance of samples to be examined in the medical laboratory.
3) Performs pre-analysis, prepares preparations, applies disinfection and sterilization techniques.
4) It has the knowledge of the preparation of the chemical materials, microbiological materials and media used for the analysis process, the methods of the patient samples taken and the methods to be applied to these materials and materials.
5) In the medical laboratory, he / she makes the medical tests required by the relevant specialist physician and submits it to the laboratory chief and / or specialist doctor to check the results and to report and approve.
6) It is responsible for the storage and storage of laboratory equipment and consumables in accordance with biosafety levels.
7) Medical laboratory equipment in accordance with the rules and techniques to use, controls and maintains the most efficient work by maintaining.
8) It follows the disposal of waste according to the biosecurity rules and provides urgent information to the responsible personnel in critical situations related to laboratory safety.
9) The organization organizes work in the medical laboratory and undertakes the responsibility of team work and takes individual responsibility when fulfilling its professional duties.
10) Establishes effective communication with colleagues, patients, physicians and other health professionals by considering professional and ethical values related to the field.
11) As an individual working in the field of medical laboratory, he acts in accordance with the laws, regulations and legislation related to his duties, rights and responsibilities.
12) Has enough awareness about individual and public health, environmental protection and occupational safety. Develops itself in communication, takes an active role in group work.
13) It has the awareness of lifelong learning and directs its education to an advanced level of education in the same field or to a profession at the same level.
14) It follows information in the field by using a foreign language at least at the level of European Language Portfolio A2 General Level.
15) Uses information and communication technologies together with computer software at the basic level of at least European Computer Driving License required by the field.

Course - Learning Outcome Relationship

No Effect	1 Lowest	2 Average	3 Highest

	Program Outcomes	Level of Contribution
1)	Medical laboratory techniques apply the basic knowledge and skills on the path to diagnosis from the patient samples, which are deemed necessary by the physicians, by gaining the competence towards the general and applied sciences of basic medicine fields such as microbiology, biochemistry, hematology and immunology for human health.
2)	It takes part in the acceptance of samples to be examined in the medical laboratory.
3)	Performs pre-analysis, prepares preparations, applies disinfection and sterilization techniques.
4)	It has the knowledge of the preparation of the chemical materials, microbiological materials and media used for the analysis process, the methods of the patient samples taken and the methods to be applied to these materials and materials.
5)	In the medical laboratory, he / she makes the medical tests required by the relevant specialist physician and submits it to the laboratory chief and / or specialist doctor to check the results and to report and approve.
6)	It is responsible for the storage and storage of laboratory equipment and consumables in accordance with biosafety levels.
7)	Medical laboratory equipment in accordance with the rules and techniques to use, controls and maintains the most efficient work by maintaining.
8)	It follows the disposal of waste according to the biosecurity rules and provides urgent information to the responsible personnel in critical situations related to laboratory safety.
9)	The organization organizes work in the medical laboratory and undertakes the responsibility of team work and takes individual responsibility when fulfilling its professional duties.
10)	Establishes effective communication with colleagues, patients, physicians and other health professionals by considering professional and ethical values related to the field.
11)	As an individual working in the field of medical laboratory, he acts in accordance with the laws, regulations and legislation related to his duties, rights and responsibilities.
12)	Has enough awareness about individual and public health, environmental protection and occupational safety. Develops itself in communication, takes an active role in group work.
13)	It has the awareness of lifelong learning and directs its education to an advanced level of education in the same field or to a profession at the same level.
14)	It follows information in the field by using a foreign language at least at the level of European Language Portfolio A2 General Level.
15)	Uses information and communication technologies together with computer software at the basic level of at least European Computer Driving License required by the field.

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Midterms	1	% 40
Final	1	% 60
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

Workload and ECTS Credit Calculation

Activities	Number of Activities	Workload
Course Hours	13	26
Study Hours Out of Class	13	52
Homework Assignments	13	26
Midterms	1	1
Final	1	1
Total Workload		106