| Pharmacy (English) | |||||
| Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 | ||
| Course Code: | PHA032 | ||||
| Course Name: | Cell Culture Techniques | ||||
| Semester: | Spring | ||||
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| Does the Course Require Work Experience?: | No | ||||
| Type of course: | Departmental Elective | ||||
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| Mode of Delivery: | Face to face | ||||
| Course Coordinator: | Dr. Öğr. Üy. BETÜL GİRAY | ||||
| Course Lecturer(s): | Dr. Öğr. Üye. Betül GİRAY | ||||
| Course Assistants: |
| Course Objectives: | To teach recombinant DNA technologies and cell culture methods and their fields of use in detail and to teach students the place and importance of these methods in the pharmaceutical industry. |
| Course Content: | The course includes key topics within the field of recombinant dna technology and cell culture methods. The curriculum entails the detailed information of basic tehniques of recombinant technology, appropriate vector selection for cloning, restriction enzymes, gene cloning, creating cDNA libraries. The course also includes informations about expression of proteins using various microorganisms, purification and determination of proteins, selection of appropriate cell culture media, and cell culture analysis. |
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The students who have succeeded in this course;
1) Will learn the basic techniques used in recombinant DNA Technologies and will be able to classify vector types. 2) Will be able to define restriction enzymes and select appropriate restriction enzymes. 3) Will be able to explain all molecular biological methods and select the appropriate method. 4) Will learn gene cloning, classify gene transfer methods.. 5) Will be able to recognize gene expression vectors and select the appropriate vector. 6) will be able to create cDNA libraries and explain screening methods, 7) Will be able to explain the production of recombinant proteins and classify them according to their usage areas. 8) Will be able to recognize cell culture methods and analyze the appropriate method. |
| Week | Subject | Related Preparation |
| 1) | Introduction to recombinant DNA technology, application areas | |
| 2) | Basic techniques used in recombinant DNA technology; obtaining and transferring the target DNA molecule | |
| 3) | Restriction enzymes, appropriate restriction enzyme selection | |
| 4) | Vectors; Plasmids, Bacteriophages, Cosmids M13 vectors and selection of suitable vectors | |
| 5) | Genomic and plasmid DNA isolation methods | |
| 6) | Imaging methods of DNA molecules, next generation sequencing | |
| 7) | Gene expression vectors; yeast vectors, mammalian cell vectors, super vectors (BAC,YAC,MAC,HAC) | |
| 8) | midterm exam | lessons notes |
| 9) | Primer design, amplification of the target region by PCR, PCR types, selection of appropriate method | |
| 10) | Gene transfer methods; chemical transformation, electroporation, gene transfer to animals, gene transfer to plants | |
| 11) | Creation and screening of genomic and cDNA libraries | |
| 12) | Posttranscriptional mechanisms in bacteria and eukaryotic cells and selection of the appropriate microorganism for recombinant protein production. | |
| 13) | Recombinant protein production | |
| 14) | Cell Culture Methods | |
| 15) | Final exam | lessons notes |
| Course Notes / Textbooks: | Hücre Kültürü ve Temel Moleküler Biyoloji Protokolleri Mustafa Arık Genetik Mühendisliği Rekombinant DNA Teknolojileri Prof. Dr. Bilge Hilal ÇADIRCI |
| References: | Recombinant DNA Technology Sardul Singh Sandhu Cell Culture Techniques Michael Aschner; Cristina Suñol; Anna K Bal-Price |
| Course Learning Outcomes | 1 |
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| Program Outcomes | |||||||||||
| 1) Applies and develops the universal and social dimensional effects of basic, professional and technological knowledge in the field of pharmacy as well as pharmacy practice interdisciplinary by following legal, deontological and ethical rules. | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |||
| 2) Defines the terminology related to the pharmacy profession; solves problems , accesses scientific information in the field of pharmacy, after monitoring and evaluating the current literature, applies , communicates, shares by using information technologies effectively and efficiently. | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
| 3) Uses theoretical and practical knowledge about the anatomical structure of the human body, the physiological working principles of systems, biochemical, immunological events in the organism and microorganisms. | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||
| 4) Uses basic and advanced analytical techniques and methods by running qualitative/quantitative analyzes and interprets the findings by using appropriate statistical methods. | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | |||
| 5) Defines medicinal plants, herbal drugs and active substances; gains the skills for the development of natural products used for medical purposes. | |||||||||||
| 6) Applies patient-centered and individualized pharmaceutical care service together with other healthcare personnel within the framework of rational drug use by using the principles of clinical pharmacy, pharmacoeconomics, pharmacotherapy and phytotherapy. | |||||||||||
| 7) Knows the biological properties, structure-activity relationships, and metabolisms of drugs and gains the skill for the synthesize and development of new drug candidates. | 2 | 1 | 1 | 1 | 2 | 2 | |||||
| 8) He/She is competent at formulations, production, stability, quality assurance, licensing, patent studies, legal regulations of products containing natural origin and / or synthetic active substances, advanced therapy medicinal products, radiopharmaceuticals and cosmetic products. | |||||||||||
| 9) Interprets the pharmacokinetic and pharmacodynamic properties of drugs, the factors that change their effect, their toxic effects, pharmacolygical activities and their risk assessment method. Reports the drug interactions and adverse drug reactions, monitors and applies the theoretical/practical knowledge for preventing them. | |||||||||||
| 10) As a health professional in his/her profession he/she acts as a caregiver, decision maker, communicator, manager, lifelong learner, instructor, leader and researcher, he/she complies with the principles of evidence-based pharmacy by making teamwork for the benefit of society, national and universal values. | |||||||||||
| 11) He/She works in various fields such as community pharmacy, hospitals, pharmaceutical medical devices, herbal products and cosmetics sectors, health institutions and agencies, clinical research organizations , universities and R&D centers. | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | |||
| No Effect | 1 Lowest | 2 Average | 3 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | Applies and develops the universal and social dimensional effects of basic, professional and technological knowledge in the field of pharmacy as well as pharmacy practice interdisciplinary by following legal, deontological and ethical rules. | 2 |
| 2) | Defines the terminology related to the pharmacy profession; solves problems , accesses scientific information in the field of pharmacy, after monitoring and evaluating the current literature, applies , communicates, shares by using information technologies effectively and efficiently. | 1 |
| 3) | Uses theoretical and practical knowledge about the anatomical structure of the human body, the physiological working principles of systems, biochemical, immunological events in the organism and microorganisms. | 1 |
| 4) | Uses basic and advanced analytical techniques and methods by running qualitative/quantitative analyzes and interprets the findings by using appropriate statistical methods. | 2 |
| 5) | Defines medicinal plants, herbal drugs and active substances; gains the skills for the development of natural products used for medical purposes. | |
| 6) | Applies patient-centered and individualized pharmaceutical care service together with other healthcare personnel within the framework of rational drug use by using the principles of clinical pharmacy, pharmacoeconomics, pharmacotherapy and phytotherapy. | |
| 7) | Knows the biological properties, structure-activity relationships, and metabolisms of drugs and gains the skill for the synthesize and development of new drug candidates. | 1 |
| 8) | He/She is competent at formulations, production, stability, quality assurance, licensing, patent studies, legal regulations of products containing natural origin and / or synthetic active substances, advanced therapy medicinal products, radiopharmaceuticals and cosmetic products. | |
| 9) | Interprets the pharmacokinetic and pharmacodynamic properties of drugs, the factors that change their effect, their toxic effects, pharmacolygical activities and their risk assessment method. Reports the drug interactions and adverse drug reactions, monitors and applies the theoretical/practical knowledge for preventing them. | |
| 10) | As a health professional in his/her profession he/she acts as a caregiver, decision maker, communicator, manager, lifelong learner, instructor, leader and researcher, he/she complies with the principles of evidence-based pharmacy by making teamwork for the benefit of society, national and universal values. | |
| 11) | He/She works in various fields such as community pharmacy, hospitals, pharmaceutical medical devices, herbal products and cosmetics sectors, health institutions and agencies, clinical research organizations , universities and R&D centers. | 3 |
| Semester Requirements | Number of Activities | Level of Contribution |
| Midterms | 1 | % 30 |
| Final | 1 | % 70 |
| total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 30 | |
| PERCENTAGE OF FINAL WORK | % 70 | |
| total | % 100 | |
| Activities | Number of Activities | Workload |
| Course Hours | 14 | 28 |
| Study Hours Out of Class | 14 | 54 |
| Midterms | 1 | 2 |
| Final | 1 | 2 |
| Total Workload | 86 | |