| Cancer Biology and Pharmacology (Master) (with Thesis) | |||||
| Master | TR-NQF-HE: Level 7 | QF-EHEA: Second Cycle | EQF-LLL: Level 7 | ||
| Course Code: | KBY5005 | ||||
| Course Name: | Cell Culture Techniques | ||||
| Semester: | Spring | ||||
| Course Credits: |
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| Language of instruction: | |||||
| Course Condition: | |||||
| Does the Course Require Work Experience?: | No | ||||
| Type of course: | Departmental Elective | ||||
| Course Level: |
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| Mode of Delivery: | |||||
| Course Coordinator: | Prof. Dr. ENGİN ULUKAYA | ||||
| Course Lecturer(s): |
Prof. Dr. ENGİN ULUKAYA |
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| Course Assistants: |
| Course Objectives: | The aim of the course is to acquire basic information about cell culture, to prepare materials and to have information about cell culture applications. |
| Course Content: | Tissue and cell culture definitions, application areas, sterile working conditions, microscopic analysis, in vitro cytotoxicity and cell death analysis. |
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The students who have succeeded in this course;
1) To be able to explain the uses and benefits of animal tissue culture 2) To be able to explain the information about the equipment, consumables and media used in cell culture techniques 3) To be able to express the characteristics of cultured cells 4) To be able to explain and apply basic cell culture techniques |
| Week | Subject | Related Preparation |
| 1) | Introduction to tissue and cell culture | |
| 2) | Biological properties of tissue and cell culture | |
| 3) | Basic cell culture - 1 | |
| 4) | Basic cell culture - 2 | |
| 5) | Primary and secondary cell culture | |
| 6) | Quality control - Contamination | |
| 7) | Quantification | |
| 8) | Cell seperation | |
| 9) | Cytotoxicity | |
| 10) | Calculation and Preparation of Drugs / Chemical Compounds | |
| 11) | Cell death | |
| 12) | Problem solution | |
| 13) | 3D cell culture | |
| 14) | General overview |
| Course Notes / Textbooks: | Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. R. Ian Freshney. 6th Edition. |
| References: |
| Course Learning Outcomes | 1 |
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| Program Outcomes | ||||||||
| 1) The ability to define basic concepts of Cancer Biology and Pharmacology, get information objectively about the related field within the scope of the program | ||||||||
| 2) Having knowledge about the molecular mechanisms of cancer, learning the process of carcinogenesis at molecular level | ||||||||
| 3) Learning of pharmacology and anti-cancer drug development, screening and relevant molecular/cellular techniques (cytotoxicity, etc.), get information about the postgraduate regulation related to cancer research and drug development, cooperation and experience share with institutions providing technological support | ||||||||
| 4) The ability to synthesize, analyze, interpret, interrogate and criticize the relevant information on cancer biology and drug development | ||||||||
| 5) The ability to use good communication skills and computer technology efficiently in their work | ||||||||
| 6) The ability to convey/present the findings of the research and the results either verbally or in writing | ||||||||
| 7) The ability to carry out professional and academic studies independently using accumulated knowledge and to work and take responsibility as a team member with other professions working in this field | ||||||||
| 8) Having a sense of ethical responsibility in research | ||||||||
| No Effect | 1 Lowest | 2 Average | 3 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | The ability to define basic concepts of Cancer Biology and Pharmacology, get information objectively about the related field within the scope of the program | 3 |
| 2) | Having knowledge about the molecular mechanisms of cancer, learning the process of carcinogenesis at molecular level | 1 |
| 3) | Learning of pharmacology and anti-cancer drug development, screening and relevant molecular/cellular techniques (cytotoxicity, etc.), get information about the postgraduate regulation related to cancer research and drug development, cooperation and experience share with institutions providing technological support | 1 |
| 4) | The ability to synthesize, analyze, interpret, interrogate and criticize the relevant information on cancer biology and drug development | 2 |
| 5) | The ability to use good communication skills and computer technology efficiently in their work | |
| 6) | The ability to convey/present the findings of the research and the results either verbally or in writing | |
| 7) | The ability to carry out professional and academic studies independently using accumulated knowledge and to work and take responsibility as a team member with other professions working in this field | |
| 8) | Having a sense of ethical responsibility in research | 3 |
| Semester Requirements | Number of Activities | Level of Contribution |
| Laboratory | 7 | % 20 |
| Midterms | 1 | % 30 |
| Final | 1 | % 50 |
| total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 50 | |
| PERCENTAGE OF FINAL WORK | % 50 | |
| total | % 100 | |
| Activities | Number of Activities | Preparation for the Activity | Spent for the Activity Itself | Completing the Activity Requirements | Workload | ||
| Course Hours | 14 | 2 | 2 | 56 | |||
| Laboratory | 14 | 2 | 2 | 2 | 84 | ||
| Midterms | 1 | 20 | 3 | 23 | |||
| Final | 1 | 30 | 3 | 33 | |||
| Total Workload | 196 | ||||||