Molecular Biology and Genetics (English) Preview
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course Introduction and Application Information

Course Code:

MBG311

Course Name:

Recombinant DNA Technology

Semester:

Fall

Course Credits:

ECTS

Language of instruction:

English

Course Condition:

Does the Course Require Work Experience?:

Type of course:

Compulsory Courses

Course Level:

Bachelor

TR-NQF-HE:6. Master`s Degree

QF-EHEA:First Cycle

EQF-LLL:6. Master`s Degree

Mode of Delivery:

Face to face

Course Coordinator:

Araş. Gör. KAAN ADACAN

Course Lecturer(s):

Dr. Öğr. Üy. ASLI KUTLU

Course Assistants:

Course Objective and Content

Course Objectives:	The aim of lesson is summarized as constituting the basic knowledge of recombinant DNA technology, to learn the details about the techniques used in this field, gaining the ability to create a workflow, and owing the knowledge of different biotechnological applications related to recombinant DNA technologies.
Course Content:	The rationality, workflows and methods of recombinant DNA technology constitute the content of the course.

Learning Outcomes

The students who have succeeded in this course;
1) Able to know the importance of recombinant DNA technology techniques in the biotechnological processes
2) Has a deep knowledge about different techniques used in recombinant DNA technology
3) Has a detailed knowledge about gene transfer techniques
4) Has a knowledge in the field of strategies required to solve recent problems with recombinant DNA technologies
5) Has a knowledge in the field of genetic engineering

Course Flow Plan

Week	Subject	Related Preparation
1)	Introduction
2)	What is biotechnology? How is it used for varied applications?
3)	Recombinant DNA technology: perspectives from genetic engineering
4)	Gene cloning approach 1
5)	Gene cloning approach 2
6)	Gene transfer approach 1
7)	Gen transfer yaklaşımı 2
8)	Midterm week
9)	DNA replication & gene expression 1
10)	DNA replication & gene expression 2
11)	Crispr CAS9 & applications
12)	CAR T-cell treatment & applications
13)	The production of recombinant DNA products: Fermentation 1
14)	The production of recombinant DNA products: Fermentation 2

Sources

Course Notes / Textbooks:	Biotechnology, 5th Edition, Cambridge; Molecular Biotechnology: Principles and Applications of Recombinant DNA 4th Edition by Glick, Pasternak and Patten "
References:	Literatürde yer alan diğer yayınlar

Course - Program Learning Outcome Relationship

Course Learning Outcomes	1	2	3	4	5
Program Outcomes
1) Has a theoretical and practical background in biology, chemistry, physics and mathematics, which constitute the basic knowledge in the field of molecular biology and genetics.
2) Can explain biological phenomena and events at molecular level and relate them to other basic sciences and engineering applications.
3) Has the basic laboratory knowledge and skills required by the field.
4) Works in accordance with scientific principles and ethical rules.
5) Uses procedural and mathematical software programs required for the analysis and basic evaluation of biological data at least at the European Computer License Basic Level.
6) Has the knowledge, culture and skills to follow the literature and current methods related to his field.
7) Will be able to identify the main problem in line with the needs in health, agriculture, animal husbandry, environment, industry and similar issues and offer the necessary solutions by using up-to-date technology.
8) Has the knowledge and ability to evaluate biological phenomena and events at the level of systems from an evolutionary point of view.
9) Has the ability to be involved in individual and group work, to prepare and carry out projects on specific topics, and to make written and oral presentations.
10) Uses at least one foreign language in reading, writing and speaking at B1 General Level in terms of European Language Portfolio criteria.
11) Has the ability to identify social and global problems using his / her field knowledge and to be a part of the solution in interdisciplinary cooperation.
12) Respects social, cultural and individual differences, universal values and human rights in his / her scientific and professional activities.

Course - Learning Outcome Relationship

No Effect	1 Lowest	2 Average	3 Highest

	Program Outcomes	Level of Contribution
1)	Has a theoretical and practical background in biology, chemistry, physics and mathematics, which constitute the basic knowledge in the field of molecular biology and genetics.
2)	Can explain biological phenomena and events at molecular level and relate them to other basic sciences and engineering applications.	3
3)	Has the basic laboratory knowledge and skills required by the field.
4)	Works in accordance with scientific principles and ethical rules.	2
5)	Uses procedural and mathematical software programs required for the analysis and basic evaluation of biological data at least at the European Computer License Basic Level.
6)	Has the knowledge, culture and skills to follow the literature and current methods related to his field.	2
7)	Will be able to identify the main problem in line with the needs in health, agriculture, animal husbandry, environment, industry and similar issues and offer the necessary solutions by using up-to-date technology.	2
8)	Has the knowledge and ability to evaluate biological phenomena and events at the level of systems from an evolutionary point of view.
9)	Has the ability to be involved in individual and group work, to prepare and carry out projects on specific topics, and to make written and oral presentations.
10)	Uses at least one foreign language in reading, writing and speaking at B1 General Level in terms of European Language Portfolio criteria.
11)	Has the ability to identify social and global problems using his / her field knowledge and to be a part of the solution in interdisciplinary cooperation.
12)	Respects social, cultural and individual differences, universal values and human rights in his / her scientific and professional activities.

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Quizzes	5	% 10
Midterms	1	% 40
Final	1	% 50
total		% 100
PERCENTAGE OF SEMESTER WORK		% 50
PERCENTAGE OF FINAL WORK		% 50
total		% 100

Workload and ECTS Credit Calculation

Activities	Number of Activities	Preparation for the Activity	Spent for the Activity Itself	Completing the Activity Requirements	Workload
Course Hours	13	0	3		39
Study Hours Out of Class	13	0	3		39
Quizzes	5	0	1		5
Midterms	1	0	10		10
Final	1	0	20		20
Total Workload					113